Journal: Frontiers in Immunology

Article Title: Paeonol Interferes With Quorum-Sensing in Pseudomonas aeruginosa and Modulates Inflammatory Responses In Vitro and In Vivo

doi: 10.3389/fimmu.2022.896874

Figure Lengend Snippet: Paeonol changed the Polarization of Macrophages infected with PAO1 (MOI = 25:1). Control (A) , PAO1 (B) The data in “PAO1+Pae 32 μg/mL” (D) , “PAO1+Pae 64 μg/mL” (E) , and “PAO1+Pae 128 μg/mL” (F) indicated that Paeonol reversed the upregulated the biomarker of CD86 of RAW264.7 cells infected with PAO1 (C) . The cell polarization was distinguished by Flow cytometry. All data were expressed as means ± SD (n=3). * P < 0.05 or ** P < 0.01 vs PAO1 group.

Article Snippet: The cells were stained with F4/80 (Elabscience, E-AB-F0995C), CD86 (Elabscience, E-AB-F0994E), and CD206 (Elabscience, E-AB-F1135D) fluorescently labeled antibody, then detected by a flow cytometer (Beckman coulter, CytoFLEX) and analyzed by the Flowjo software.

Techniques: Infection, Control, Biomarker Discovery, Flow Cytometry

Journal: PLoS ONE

Article Title: Sulforaphane Epigenetically Regulates Innate Immune Responses of Porcine Monocyte-Derived Dendritic Cells Induced with Lipopolysaccharide

doi: 10.1371/journal.pone.0121574

Figure Lengend Snippet: List of primer sequences used in this study.

Article Snippet: Cells were stained with a mouse anti-human CD40 FITC Ab (clone G28.5, NB100-77786, Novus Biologicals), a mouse anti-human CD80 PE Ab (clone 37711, FAB140P, R&D systems) and a mouse anti-human CD86 APC Ab (clone 37301, FAB141A, R&D systems) antibodies.

Techniques: Amplification

Journal: PLoS ONE

Article Title: Sulforaphane Epigenetically Regulates Innate Immune Responses of Porcine Monocyte-Derived Dendritic Cells Induced with Lipopolysaccharide

doi: 10.1371/journal.pone.0121574

Figure Lengend Snippet: moDCs at day 7 in culture were used for cell phagocytosis and cell differentiation status analysis. moDCs were pre-incubated for 1 h with or without SFN (10 μM) before stimulation for 24 h LPS (1.0 μg/ml) or to the indicated concentrations. CD40, CD80, and CD86 cellular surface markers expression were analyzed by flow cytometry (A). The flow cytometry results shown were from one experiment of two independent experiments. CD40, CD80 and CD86 mean fluorescence intensity (MFI) determined by flow cytometry (B). The flow cytometry results were combined from two independent experiments and each experiment was performed from triplications. Data are mean ± standard deviations (SD) (the letters a and b P< 0.01). The phagocytic activity of moDCs was examined after stimulating with different concentration of LPS (0,5 μg/ml, 1,0 μg/ml, and 2,0 μg/ml) with or without 24 h pre-treatment with SFN (C). The mRNA expression of DCs surface markers CD40, CD80 and CD86 were quantified using qRT-PCR (D). The mRNA expression and phagocytosis results were combined from three independent experiments and each experiment was performed in four replications. The data represented as the mean ± standard deviations (SD) (* P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: Cells were stained with a mouse anti-human CD40 FITC Ab (clone G28.5, NB100-77786, Novus Biologicals), a mouse anti-human CD80 PE Ab (clone 37711, FAB140P, R&D systems) and a mouse anti-human CD86 APC Ab (clone 37301, FAB141A, R&D systems) antibodies.

Techniques: Cell Differentiation, Incubation, Expressing, Flow Cytometry, Fluorescence, Activity Assay, Concentration Assay, Quantitative RT-PCR